Details about the neuroanatomical projections from SS-bfd to PG
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Report ID | Sending structure | Receiving structure | Projection strength | Technique | General description projection | Collator | Associated reference |
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38943 | Primary somatosensory area barrel field | Pontine gray | light/moderate | biotinylated dextran amine (BDA) | The BDA and FR clusters in case D46 were partially overlapping at practically all rostrocaudal or mediolateral levels containing labeling (Figs. 3D, 4, 7A,B). The total amount of overlap in this case was 12.1%. The separation of the two injections was 930 µm. In another within row experiment (D53), the separation distance between the injection sites was much larger (1700 µm). Although the same overall shape and lamellar distribution pattern was seen in this case, the labeled clusters were clearly more segregated (Fig. 7C,D), and the amount of total corticopontine overlap was only 1.7%. Five within row experiments demonstrated decreasing amount of overlap with increasing separation of the cortical sites of origin (Table 1, Fig. 8).
| Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38944 | Primary somatosensory area barrel field | Pontine gray | strong | biotinylated dextran amine (BDA) | The BDA and FR clusters in case D46 were partially overlapping at practically all rostrocaudal or mediolateral levels containing labeling (Figs. 3D, 4, 7A,B). The total amount of overlap in this case was 12.1%. The separation of the two injections was 930 µm. In another within row experiment (D53), the separation distance between the injection sites was much larger (1700 µm). Although the same overall shape and lamellar distribution pattern was seen in this case, the labeled clusters were clearly more segregated (Fig. 7C,D), and the amount of total corticopontine overlap was only 1.7%. Five within row experiments demonstrated decreasing amount of overlap with increasing separation of the cortical sites of origin (Table 1, Fig. 8). | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38945 | Primary somatosensory area barrel field | Pontine gray | light/moderate | FluoroRuby | The BDA and FR clusters in case D46 were partially overlapping at practically all rostrocaudal or mediolateral levels containing labeling (Figs. 3D, 4, 7A,B). The total amount of overlap in this case was 12.1%. The separation of the two injections was 930 µm. In another within row experiment (D53), the separation distance between the injection sites was much larger (1700 µm). Although the same overall shape and lamellar distribution pattern was seen in this case, the labeled clusters were clearly more segregated (Fig. 7C,D), and the amount of total corticopontine overlap was only 1.7%. Five within row experiments demonstrated decreasing amount of overlap with increasing separation of the cortical sites of origin (Table 1, Fig. 8). | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38946 | Primary somatosensory area barrel field | Pontine gray | strong | FluoroRuby | The BDA and FR clusters in case D46 were partially overlapping at practically all rostrocaudal or mediolateral levels containing labeling (Figs. 3D, 4, 7A,B). The total amount of overlap in this case was 12.1%. The separation of the two injections was 930 µm. In another within row experiment (D53), the separation distance between the injection sites was much larger (1700 µm). Although the same overall shape and lamellar distribution pattern was seen in this case, the labeled clusters were clearly more segregated (Fig. 7C,D), and the amount of total corticopontine overlap was only 1.7%. Five within row experiments demonstrated decreasing amount of overlap with increasing separation of the cortical sites of origin (Table 1, Fig. 8). | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38947 | Primary somatosensory area barrel field | Pontine gray | moderate | biotinylated dextran amine (BDA) | When BDA and FR injections were placed in different rows, the ensuing labeled terminal fields had a lamellar shape as described above. Instead of producing a dual lamellar pattern, however, the labeled clusters for both tracers appeared to be localized within a single lamellar volume. Case D48 illustrates this finding in both sagittal and transverse slices (Fig. 5B, slices 50-80; 5C, slices 60-80). The BDA labeling arising from a barrel in row D tended to extend more ventrally in the pontine nuclei than the FR labeling arising in an adjacent barrel in row C (Figs. 5B, slices 60-80, 6D-F). These topographic differences however, were less conspicuous than the marked inside-out pattern observed in the within row cases. In case D48, the injection sites were separated by only 120 µm in cortical layer V, and total corticopontine overlap was 14.5% (Fig. 7E,F). | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38948 | Primary somatosensory area barrel field | Pontine gray | moderate | FluoroRuby | When BDA and FR injections were placed in different rows, the ensuing labeled terminal fields had a lamellar shape as described above. Instead of producing a dual lamellar pattern, however, the labeled clusters for both tracers appeared to be localized within a single lamellar volume. Case D48 illustrates this finding in both sagittal and transverse slices (Fig. 5B, slices 50-80; 5C, slices 60-80). The BDA labeling arising from a barrel in row D tended to extend more ventrally in the pontine nuclei than the FR labeling arising in an adjacent barrel in row C (Figs. 5B, slices 60-80, 6D-F). These topographic differences however, were less conspicuous than the marked inside-out pattern observed in the within row cases. In case D48, the injection sites were separated by only 120 µm in cortical layer V, and total corticopontine overlap was 14.5% (Fig. 7E,F). | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38949 | Primary somatosensory area barrel field | Pontine gray | strong | biotinylated dextran amine (BDA) | In another across row case (D51), the two injection sites were separated by 630 µm (Fig. 7G,H). The labeled clusters in D51 were also confined within a lamellar subspace. The clusters appeared to be more segregated than in case D48, as indicated by a reduction in the total amount of corticopontine overlap (9.6%). As Table 1 indicates, we observed decreasing amounts of corticopontine overlap with increasing separation of the tracer injection sites (Table 1, Fig. 8).
| Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38950 | Primary somatosensory area barrel field | Pontine gray | strong | FluoroRuby | In another across row case (D51), the two injection sites were separated by 630 µm (Fig. 7G,H). The labeled clusters in D51 were also confined within a lamellar subspace. The clusters appeared to be more segregated than in case D48, as indicated by a reduction in the total amount of corticopontine overlap (9.6%). As Table 1 indicates, we observed decreasing amounts of corticopontine overlap with increasing separation of the tracer injection sites (Table 1, Fig. 8). | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38951 | Primary somatosensory area barrel field | Pontine gray | moderate | biotinylated dextran amine (BDA) | Collator note: see Figure 7 page 8480 and Table 1 page 8475. | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38952 | Primary somatosensory area barrel field | Pontine gray | light | FluoroRuby | Collator note: see Figure 7 page 8480 and Table 1 page 8475. | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38953 | Primary somatosensory area barrel field | Pontine gray | strong | biotinylated dextran amine (BDA) | Collator note: see Figure 7 page 8480 and Table 1 page 8475. | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 | |
38954 | Primary somatosensory area barrel field | Pontine gray | moderate | FluoroRuby | Collator note: see Figure 7 page 8480 and Table 1 page 8475. | Leergaard T.B., Alloway K.D., Mutic J.J. & Bjaalie J.G., 2000 |
General description technique/protocol: In each experiment, restricted injections of BDA and FR were placed into separate regions of the SI barrel cortex (Fig. 1). Ten of the 13 experiments were selected by criterion of labeling in the neostriatum and thalamus and were used previously in a study of corticostriatal organization (Alloway et al., 1999). All cases contained anterograde BDA and FR labeling in the pontine nuclei. All injections were restricted to the gray matter. The outlines and position of the injection sites were estimated with respect to the whisker barrels by comparison of adjacent BDA- and CO-processed tangential sections through cortical layers IV and V. The position of injections in the whisker barrel field corresponded to the electrophysiological recordings made before tracer injection. The diameter of the injection sites (Table 1), defined by the maximum width of dense staining in layer V (the cortical layer containing cell bodies of corticopontine neurons), ranged from 200 to 800 µm. Each injection involved one to two barrels (Table 1). In five cases, both tracers were confined to the same row of barrels ("within rows"), and in eight cases the tracers were injected into separate rows of barrels ("across rows"). The edge-to-edge separation between the two injection sites was measured in layer V (Fig. 2). Injection sites never overlapped. Photomicrographs of representative injection sites are shown in Figure 2. |
Further details associated to record number 38949:
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