Case pg307-308, fig12. Soma notes 2 adult male hooded rats and 5 adult male albino rats were used. each received an injection of CT-HRP into the posterior chamber of the eye.. Terminal notes retinohypothalamic tract 3 components: 1) largest is innervation of SCN. Rostral SCN: a very dense band of terminals at border of SCN and optic chiasm, Mid SCN: most of ven.lat. component of nuc filled with dense terminal plexus, remainder less dense.

Case pg348,349-50, fi3,6. Soma notes 36 female Sprague-Dawley rats weighing 200-350g. 10 microliters of CT-HRP(.20%-.40%) were injected into one eye behind the lens into the vitreous chamber of the eye. pressure injection over 1 min. Terminal notes Medial component of the RHTa) inbtwn 2 SCNs under v3, b) ven. lat. to SCN, appear to cradle it high density of terminal labeling in and around SCN. (ven.lat. parts of ros. SCN, venlat. dorlat. parts of cau. SCN.) dormed. subncl of SCN largely spared..

Case pg3, fig1. Soma notes 30 animals used for autoradiography study. Each received 1 injection of either L-leucine-3H or L-proline-3H into the posterior chamber of one eye. Terminal notes The autoradiographic material provided substantial evidence for a retinohypothalamic tract. The hypothalamic nuclei themselves were free of labelling save for the suprachiasmatic nucleus, both ipsi and contra, especially in ventral 1/2.

Case pg237, fig6,7. Soma notes Both these expts (E30 & E33) produced heavy retrograde labeling in the region of the SCh.. Terminal notes Expt E33 involved a smaller liquid injection (than E30) in the paraventricular nucleus of the thalamus.

Case pg208,211, fig3H-N. Soma notes 3 PHA-L injs were virtually limited to the SCh, with less than 5% of labeled neurons outside boundaries of nucleus itself. Injs A7 & A54 tended to label cells in ventral part of nucleus, while inj A25 extended more dorsally. Terminal notes 3rd Pway. pathway courses ventrally to innervate the preoptic continuation of the bed nucelus as well as parataenial & paraventricular nuc of thalamus. In PT & PVT fibres are thin, widely spaced small varicosities and give rise to many terminal boutons..

Case pg208,212, fig3I-Q. Soma notes 3 PHA-L injs were virtually limited to the SCh, with less than 5% of labeled neurons outside boundaries of nucleus itself. Injs A7 & A54 tended to label cells in ventral part of nucleus, while inj A25 extended more dorsally. Terminal notes 5th Pway. Many fibres from PVH appear to continue dorsally through midline thalamic nuclei ( reuniens, rhomboid & central medial) to end in more caudal parts of the PVT. Some branching/terminal boutons seen in PVT, much less freq in RE & RH..

ipsilateral, earlier: Berk & Finkelstein Brain Res. 226:1-13, 1981, rat, autoradiography

Case pg76, fig3. Soma notes PHA-L was injected in the paraventricular nuc ?bilaterally. --See fig 1 for examples of inj sites, but not for every case-. The diameter of the inj sites varies from 150 to 600u. PHA-L was injected at various rostrocaudal levels of paraventricular nuc. Terminal notes More dense --Than in PRh-- anterograde label is present in the ventral subiculum, where the labelled fibres are organized in 2 bands. one of which is located in the molecular layer and the other in the pyramidal cell layer.

Collator note: labeling of the LHA, just above the optic tract. See Fig. 3A, Swanson Atlas Levels 26-27.