Experiment acronym: Watts-CRH/AVP
Data types: quantitative
Mapping approach:
brain region is captured in original published nomenclature, in BAMS
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
representative drawings/mappings onto templates
| Animals (subjects):
Number: 5 Sex: not specified
Age: not specified Weight: 280-320g
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: stain specific
Staining frequency: 1:8
Technique: in situ hybridization
| Measurement 1:
Measured nucleic acid: mRNA
Source (producer): Strategeme Corp
Probe sequence: 700 bp RsaI-RsaI CRH
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: not specified
Visualization method: DIG
Visualization medium: slide
Annotation: ....a 700 bp RsaI-RsaI fragment of the rat CRH gene (rCRH1) corresponding to part of the 3 end of exon 1 and the entire translated region of exon 2 (Frim et al., 1990) was subcloned into Bluescript (Stratagene Corp., La Jolla, CA) and linearized with EcoRl to generate an antisense digoxigenin-UTP labelled ppCRH cRNA probe...Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization..Digoxigenin-UTP was visualized with the reagents from the digoxigenin nucleic acid detection kit (The Genius System, Boehringer-Mannheim Corp.).
| Measurement 2:
Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: cDNA AVP 1004
Sequence species:
Probe sequence orientation: antisense
Control: sense
Labelling method: not specified
Visualization method: autoradiogram
Visualization medium: slide
Annotation: The pp- AVP sequence was generated from the cDNA AW 1004 described by Carrazana et al. (1988)....Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization..
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| Experiment acronym: Watts-CRH/NT
Data types: quantitative
Mapping approach:
brain region is captured in original published nomenclature, in BAMS
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
representative drawings/mappings onto templates
| Animals (subjects):
Number: 5 Sex: not specified
Age: not specified Weight: 280-320g
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: stain specific
Staining frequency: 1:8
Technique: in situ hybridization
| Measurement 1:
Measured nucleic acid: mRNA
Source (producer): Strategeme Corp
Probe sequence: 700 bp RsaI-RsaI CRH
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: DIG
Visualization medium: slide
Annotation: ....a 700 bp RsaI-RsaI fragment of the rat CRH gene (rCRH1) corresponding to part of the 3 end of exon 1 and the entire translated region of exon 2 (Frim et al., 1990) was subcloned into Bluescript (Stratagene Corp., La Jolla, CA) and linearized with EcoRl to generate an antisense digoxigenin-UTP labelled ppCRH cRNA probe...Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization..Digoxigenin-UTP was visualized with the reagents from the digoxigenin nucleic acid detection kit (The Genius System, Boehringer-Mannheim Corp.).
| Measurement 2:
Measured nucleic acid: mRNA
Source (producer): Promega Gemini
Probe sequence: pp-neurotensin/neuromedin
Sequence species:
Probe sequence orientation: antisense
Control: sense
Labelling method: not specified
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Sections were hybridized with 35S UTP- labelled complementary RNA (cRNA) probes transcribed from cDNA sequences for part of the mRNAs encoding pp-neurotensin/neuromedin N (NT/N), or pp-vasopressin...Details of the probes and their
characterization have been given in publications from this and other laboratories (Carrazana et al., 1988; Swanson and Simmons, 1989; Frim et al., 1990; Watts, 1992b).. Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization...All 35S-UTP probes were synthesized using the Riboprobe Gemini System I1 kit (Promega Inc., Madison, WI) and the appropriate RNA polymerase using a modification of the method of Melton et al. (1984)
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| Experiment acronym: Watts-CRH/pENK
Data types: quantitative
Mapping approach:
brain region is captured in original published nomenclature, in BAMS
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
representative drawings/mappings onto templates
| Animals (subjects):
Number: 5 Sex: not specified
Age: not specified Weight: 280-320g
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: morphology
Staining frequency: 1:8
Technique: in situ hybridization
| Measurement 1:
Measured nucleic acid: mRNA
Source (producer): Stratageme Corp
Probe sequence: 700 bp RsaI-RsaI CRH
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: DIG
Visualization medium: slide
Annotation: ....a 700 bp RsaI-RsaI fragment of the rat CRH gene (rCRH1) corresponding to part of the 3 end of exon 1 and the entire translated region of exon 2 (Frim et al., 1990) was subcloned into Bluescript (Stratagene Corp., La Jolla, CA) and linearized with EcoRl to generate an antisense digoxigenin-UTP labelled ppCRH cRNA probe...Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization..Digoxigenin-UTP was visualized with the reagents from the digoxigenin nucleic acid detection kit (The Genius System, Boehringer-Mannheim Corp.).
| Measurement 2:
Measured nucleic acid: mRNA
Source (producer): Promega Gemini
Probe sequence: proenkephalin
Sequence species:
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Sections were hybridized with 35SUTP- labelled complementary RNA (cRNA) probes transcribed from cDNA sequences for part of the mRNAs encoding proenkephalin (pENK)....All 35S-UTP probes were synthesized using the Riboprobe Gemini System I1 kit (Promega Inc., Madison, WI) and the appropriate RNA polymerase using a modification of the method of Melton et al. (1984)...
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