| Animals (subjects) | | Sex: M | | Number of animals: not specified | | Age: not specified | | Mass: 200-300 | | Unit of mass: | | Housing conditions: not specified | | Annotation: Adult male Sprague-Dawley rats (200-300 g) and pups were deeply anesthetized with ether and then sacrificed by decapitation. At least three animals of each age were used for in situ hybridization histochemistry. |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: morphology | | Staining frequency: not specified |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: BGIII fragment of residues 1023-2063
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: for NMDAR1, the 1,600 base pair (bp) BglII fragment of residues 1,023-2,623 (nucleotide residues are numbered, beginning with the first reside of the initiation ATG codon)...In order to estimate the level of nonspecific bindings, we used a 100 molar excess of the cold antisense probes coexisting with the labeled probes. The cold antisense riboprobes were synthesized similarly with unlabeled nucleotides.
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