Metadata

Experiment acronym Annotation Collator Author checked
Akazawa-NMDAIn situ hybridization study of different NMDA receptor subunits expression in the rat cerebellar cortex.Mihail Botano

Animals (subjects)
Sex: M
Number of animals: not specified
Age: not specified
Mass: 200-300
Unit of mass:
Housing conditions: not specified
Annotation: Adult male Sprague-Dawley rats (200-300 g) and pups were deeply anesthetized with ether and then sacrificed by decapitation. At least three animals of each age were used for in situ hybridization histochemistry.
Experimental method
Experiment type: in situ hybridization
Neuron/glia identification method: morphology
Staining frequency: not specified

Experimental details:
Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: EcoRV1-SacI fragment of resiudes 2548-3824
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: for NMDAR2D, the 1,276-bp EcoRV-Sac1 fragment of residues 2548-3824...The cold antisense riboprobes were synthesized similarly with unlabeled nucleotides.

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: vibratome Preservation: freezing Thickness: 10 micrometer
Staining type: not specified Sampling: not specified Annotation: ...sections were cut at 10micrometers on a cryostat (-18T) and thaw-mounted onto poly-L-lysine-coated slides.

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
Mapping approach: brain region is captured in a BAMS nomenclature other than that used in the original publication