Reference: Swanson LW, Sawchenko PE, Rivier J, Vale WW, 1983: Experiment acronym: Swanson-1983(CRH)
Data types: quantitative
Mapping approach:
brain region is captured in a BAMS nomenclature other than that used in the original publication
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
drawings/template mappings of all sections
| Animals (subjects):
Number: 10 Sex: not specified
Age: not specified Weight: not specified
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: not specified
Staining frequency: 1:4 or 1:8
Technique: immunohistochemistry
| Measurement 1:
Antigen: not specified
Antigen species: not specified
Source (producer): not specified
Primary antibody: not specified
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): polyclonal
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: not specified
Visualization method: fluorescence
Visualization medium: slide
Annotation: Seven different antisera to synthetic ovine CRF (or to a fragment of CRF), which were raised in rabbits [7], were tested and all but one were found to stain cells and fibers in the rat. Since antiserum 24 ([Try22. Gly23]-CRF (1-23) conjugated to human alpha-globulin with bis-diazetized benzidine] has been found to interact with rat CRF particularly well in radioimmunoassays [Vale, unpublished observations], it was used extensively for the mapping studies reported here.
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| Experiment acronym: PS439
Data types: quantitative
qualitative
Mapping approach:
brain region is captured in a BAMS nomenclature other than that used in the original publication
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
drawings/template mappings of all sections
| Animals (subjects):
Number: 3 Sex: not specified
Age: not specified Weight: not specified
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: not specified
Staining frequency: 1:4 or 1:8
Technique: immunohistochemistry
| Measurement 1:
Antigen: CRF
Antigen species: sheep
Source (producer): not specified
Primary antibody: 24 ([Try22. Gly23]-CRF (1-23)
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): polyclonal
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: Alpha globulin
Control: not specified
Visualization method: fluorescence
Visualization medium: slide
Annotation: Seven different antisera to synthetic ovine CRF (or to a fragment of CRF), which were raised in rabbits [7], were tested and all but one were found to stain cells and fibers in the rat. Since antiserum 24 ([Try22. Gly23]-CRF (1-23) conjugated to human alpha-globulin with bis-diazetized benzidine] has been found to interact with rat CRF particularly well in radioimmunoassays [Vale, unpublished observations], it was used extensively for the mapping studies reported here.
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| Reference: Watts, A.G. & Sanchez-Watts, G., 1995: Experiment acronym: Watts-CRH/AVP(PEG)
Data types: quantitative
qualitative
Mapping approach:
brain region is captured in original published nomenclature, in BAMS
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
representative drawings/mappings onto templates
| Animals (subjects):
Number: 6 Sex: not specified
Age: not specified Weight: 280-320g
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: stain specific
Staining frequency: 1:8
Technique: in situ hybridization
| Measurement 1:
Measured nucleic acid: mRNA
Source (producer): Stratageme Corp
Probe sequence: 700 bp RsaI-RsaI CRH
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: DIG
Visualization medium: slide
Annotation: ....a 700 bp RsaI-RsaI fragment of the rat CRH gene (rCRH1) corresponding to part of the 3 end of exon 1 and the entire translated region of exon 2 (Frim et al., 1990) was subcloned into Bluescript (Stratagene Corp., La Jolla, CA) and linearized with EcoRl to generate an antisense digoxigenin-UTP labelled ppCRH cRNA probe...Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization..Digoxigenin-UTP was visualized with the reagents from the digoxigenin nucleic acid detection kit (The Genius System, Boehringer-Mannheim Corp.).
| Measurement 2:
Measured nucleic acid: mRNA
Source (producer): Promega Gemini
Probe sequence: cDNA AVP 1004
Sequence species:
Probe sequence orientation: antisense
Control: sense
Labelling method: not specified
Visualization method: autoradiogram
Visualization medium: slide
Annotation: The pp- AVP sequence was generated from the cDNA AW 1004 described by Carrazana et al. (1988)....Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization..
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| Reference: Watts A.G. & Sanchez-Watts G., 2002: Experiment acronym: Watts-CRH (PEG)
Data types: quantitative
qualitative
Mapping approach:
brain region is captured in original published nomenclature, in BAMS
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
| Animals (subjects):
Number: not specified Sex: not specified
Age: not specified Weight: 225-250g
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: stain specific
Staining frequency: 1:8
Technique: in situ hybridization
| Measurement 1:
Measured nucleic acid: mRNA
Source (producer): Promega Gemini
Probe sequence: preproCRH
Sequence species:
Probe sequence orientation: antisense
Control: not specified
Labelling method: not specified
Visualization method: autoradiogram
Visualization medium: film
Annotation: Sections were hybridized with 35S-UTP-labeled cRNA probes transcribed from cDNA sequences encoding RNAs for parts of the following genes: preproCRH (700 bp)...
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