Reference: Broberger C., De Lecea L., Sutcliffe J.G. & Hokfelt T., 1998: Experiment acronym: Broberger-H/O-MCH
Data types: quantitative
Mapping approach:
brain region is captured in original published nomenclature, in BAMS
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
representative images with label and drawn boundaries
representative drawings/mappings onto templates
| Animals (subjects):
Number: 10 Sex: not specified
Age: not specified Weight: 250-300g
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: stain specific
Staining frequency: 1:10
Technique: in situ hybridization
| Measurement 1:
Measured nucleic acid: mRNA
Source (producer): Scandinavian Gene Synthesis, Koping, Sweden).
Probe sequence: 479-527 MCH nucleotides
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Probes complementary to nucleotides 479-527 of the rat MCH mRNA(Nahon et al., 1989) and to nucleotides 2-49 of the rat H/O mRNA(Sakurai et al., 1998) were synthesized (Scandinavian Gene Synthesis, Ko¨ping, Sweden). Probe sequences were controlled against other sequences in the GenBank database, and no homologies exceeding 75% were found.
| Measurement 2:
Measured nucleic acid: mRNA
Source (producer): Scandinavian Gene Synthesis, Koping, Sweden).
Probe sequence: 2-49 H/O nucleotides
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Probes complementary to nucleotides 479-527 of the rat MCH mRNA(Nahon et al., 1989) and to nucleotides 2-49 of the rat H/O mRNA(Sakurai et al., 1998) were synthesized (Scandinavian Gene Synthesis, Ko¨ping, Sweden). Probe sequences were controlled against other sequences in the GenBank database, and no homologies exceeding 75% were found.
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| Reference: Abrahamson E.E. & Moore R.Y., 2001: Experiment acronym: Abrahamson-PH
Data types: quantitative
Mapping approach:
brain region is captured in original published nomenclature, in BAMS
Mapping details
Coordinates: none
Anatomical data presentation:
text
images of all labeled sections
drawings/template mappings of all sections
| Animals (subjects):
Number: 45 Sex: not specified
Age: not specified Weight: 300-250g
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: stain specific
Staining frequency: 1:10
Technique: immunohistochemistry
| Measurement 1:
Antigen: MCH
Antigen species: N/a
Source (producer): Sawchenko
Primary antibody: MCH antiserum
Primary antibody species: not specified
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: not specified
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Material for the analysis of the chemoarchitecture of the PHA was prepared by incubating coronal series (n=10) in antisera generated against neuropeptide Y (Peninsula, CA), met-enkephalin (Incstar, Stillwater, MN), tyrosine hydroxylase (Eugene Tech, Ramsey, NJ), gamma-aminobytyric acid (Eugene Tech), glutamic acid decarboxylase (Eugene Tech), glutamate (Sigma), 5-hydroxy-tryptamine (Incstar), dopamine-b-hydroxylase (Incstar), melanin concentrating hormone (Sawchenko), or hypocretin (van den Pol).
| Measurement 2:
Antigen: H/O
Antigen species: N/A
Source (producer): van der Pol
Primary antibody: hypocretin antiserum
Primary antibody species: not specified
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: Material for the analysis of the chemoarchitecture of the PHA was prepared by incubating coronal series (n=10) in antisera generated against neuropeptide Y (Peninsula, CA), met-enkephalin (Incstar, Stillwater, MN), tyrosine hydroxylase (Eugene Tech, Ramsey, NJ), gamma-aminobytyric acid (Eugene Tech), glutamic acid decarboxylase (Eugene Tech), glutamate (Sigma), 5-hydroxy-tryptamine (Incstar), dopamine-b-hydroxylase (Incstar), melanin concentrating hormone (Sawchenko), or hypocretin (van den Pol).
| Measurement 3:
Antigen: tyrosine hydroxilase
Antigen species: N/A
Source (producer): Eugene Tech
Primary antibody: tyrosine hydroxylase antiserum
Primary antibody species: not specified
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: Material for the analysis of the chemoarchitecture of the PHA was prepared by incubating coronal series (n=10) in antisera generated against neuropeptide Y (Peninsula, CA), met-enkephalin (Incstar, Stillwater, MN), tyrosine hydroxylase (Eugene Tech, Ramsey, NJ), gamma-aminobytyric acid (Eugene Tech), glutamic acid decarboxylase (Eugene Tech), glutamate (Sigma), 5-hydroxy-tryptamine (Incstar), dopamine-b-hydroxylase (Incstar), melanin concentrating hormone (Sawchenko), or hypocretin (van den Pol).
| Measurement 4:
Antigen: met-enkephalin
Antigen species: N/A
Source (producer): Incstar, Stillwater, MN
Primary antibody: enkephalin antiserum
Primary antibody species: not specified
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: Material for the analysis of the chemoarchitecture of the PHA was prepared by incubating coronal series (n=10) in antisera generated against neuropeptide Y (Peninsula, CA), met-enkephalin (Incstar, Stillwater, MN), tyrosine hydroxylase (Eugene Tech, Ramsey, NJ), gamma-aminobytyric acid (Eugene Tech), glutamic acid decarboxylase (Eugene Tech), glutamate (Sigma), 5-hydroxy-tryptamine (Incstar), dopamine-b-hydroxylase (Incstar), melanin concentrating hormone (Sawchenko), or hypocretin (van den Pol).
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