Experiments comparison

Reference: Sato K., Kiyama H. & Tohyama M., 1993:
Experiment acronym: Sato-AMPA
Data types:
   qualitative
Mapping approach:
   brain region is captured in a BAMS nomenclature other than that used in the original publication
Mapping details
Coordinates: none
Anatomical data presentation:
   text
   images of all labeled sections
Animals (subjects):
   Number: 3   Sex: not specified
   Age: not specified   Weight: 150g
   Housing conditions: not specified
   Annotation: not specified
Method:
Neuron/glia identification method: morphology
Staining frequency: not specified
Technique: in situ hybridization
Measurement 1:
Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: not specified
Sequence species:
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: colorimetry
Visualization medium: film
Annotation: Collator note: information about the procedure used for in situ hybridization was described in Noguchi et al. 1988 and Keinanen et al. 1990.
Reference: Jaarsma D., Wenthold R.J. & Mugnaini E., 1995:
Experiment acronym: Jaarma-Glu
Data types:
   qualitative
Mapping approach:
   brain region is captured in a BAMS nomenclature other than that used in the original publication
Mapping details
Coordinates: none
Anatomical data presentation:
   text
   representative labeled images
Animals (subjects):
   Number: 16   Sex: not specified
   Age: not specified   Weight: 100-300g
   Housing conditions: not specified
   Annotation: normal housing
Method:
Neuron/glia identification method: morphology
Staining frequency: not specified
Technique: immunohistochemistry
Measurement 1:
Antigen: Glur1-ctemrs
Antigen species: rat
Source (producer): not specified
Primary antibody: AMPA Ab7
Primary antibody species: rabbit/mouse
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: anti mouse/rabbit antibody
Secondary antibody species: goat/horse
Immunoglobulin class: Rb IgG
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: The primary antibodies employed in this study have been extensively characterized in previous reports, and their specificity has been determined (Petralia and Wenthold, 1992; Wenthold et al., 1992, 1994; Brose et al., 1993; Huntley et al., 1993; Petralia et al., 1994a,b; Siegel et al., 1994). Goat anti-rabbit and goat anti-mouse sera and rabbit and mouse PAP were purchased from Sternberger Monoclonals Inc. (Baltimore, MD). Collator note: see Table 1 page 147 for details.
Measurement 2:
Antigen: NR1 c-term
Antigen species: rat
Source (producer): not specified
Primary antibody: AbT3-1
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: anti rabbit
Secondary antibody species: goat
Immunoglobulin class: IgG
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: The primary antibodies employed in this study have been extensively characterized in previous reports, and their specificity has been determined (Petralia and Wenthold, 1992; Wenthold et al., 1992, 1994; Brose et al., 1993; Huntley et al., 1993; Petralia et al., 1994a,b; Siegel et al., 1994). AbT3-1 is a rabbit antiserum raised against the carboxy-terminus of NR1 (Petralia et al., 1994a) and recognizes the NR1-splice variants without 3’-end deletion 2, i.e., NR1-la, -2a, -lb, -2b (Sucher et al., 1992; Hollman et al., 1993; Petralia et al., 1994a). Goat anti-rabbit and goat anti-mouse sera and rabbit and mouse PAP were purchased from Sternberger Monoclonals Inc. (Baltimore, MD). Collator note: see Table 1 page 147 for details.