Reference: Jaarsma D., Wenthold R.J. & Mugnaini E., 1995: Experiment acronym: Jaarma-Glu
Data types: qualitative
Mapping approach:
brain region is captured in a BAMS nomenclature other than that used in the original publication
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
| Animals (subjects):
Number: 16 Sex: not specified
Age: not specified Weight: 100-300g
Housing conditions: not specified
Annotation: normal housing | Method:
Neuron/glia identification method: morphology
Staining frequency: not specified
Technique: immunohistochemistry
| Measurement 1:
Antigen: Glur1-ctemrs
Antigen species: rat
Source (producer): not specified
Primary antibody: AMPA Ab7
Primary antibody species: rabbit/mouse
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: anti mouse/rabbit antibody
Secondary antibody species: goat/horse
Immunoglobulin class: Rb IgG
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: The primary antibodies employed in this study have been extensively characterized in previous reports, and their specificity has been determined (Petralia and Wenthold, 1992; Wenthold et al., 1992, 1994; Brose et al., 1993; Huntley et al., 1993; Petralia et al., 1994a,b; Siegel et al., 1994). Goat anti-rabbit and goat anti-mouse sera and rabbit and mouse PAP were purchased from Sternberger Monoclonals Inc. (Baltimore, MD). Collator note: see Table 1 page 147 for details.
| Measurement 2:
Antigen: NR1 c-term
Antigen species: rat
Source (producer): not specified
Primary antibody: AbT3-1
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: anti rabbit
Secondary antibody species: goat
Immunoglobulin class: IgG
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: The primary antibodies employed in this study have been extensively characterized in previous reports, and their specificity has been determined (Petralia and Wenthold, 1992; Wenthold et al., 1992, 1994; Brose et al., 1993; Huntley et al., 1993; Petralia et al., 1994a,b; Siegel et al., 1994). AbT3-1 is a rabbit antiserum raised against the carboxy-terminus of NR1 (Petralia et al., 1994a) and recognizes the NR1-splice variants without 3’-end deletion 2, i.e., NR1-la, -2a, -lb, -2b (Sucher et al., 1992; Hollman et al., 1993; Petralia et al., 1994a). Goat anti-rabbit and goat anti-mouse sera and rabbit and mouse PAP were purchased from Sternberger Monoclonals Inc. (Baltimore, MD). Collator note: see Table 1 page 147 for details.
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| Reference: Akazawa C., Shigemoto R., Bessho Y., Nakanishi S. & Mizuno N., 1994: Experiment acronym: Akazawa-NMDA
Data types: qualitative
Mapping approach:
brain region is captured in a BAMS nomenclature other than that used in the original publication
Mapping details
Coordinates: none
Anatomical data presentation:
text
representative labeled images
| Animals (subjects):
Number: not specified Sex: not specified
Age: not specified Weight: 200-300g
Housing conditions: not specified
Annotation: not specified | Method:
Neuron/glia identification method: morphology
Staining frequency: not specified
Technique: in situ hybridization
| Measurement 1:
Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: BGIII fragment of residues 1023-2063
Sequence species:
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: for NMDAR1, the 1,600 base pair (bp) BglII fragment of residues 1,023-2,623 (nucleotide residues are numbered, beginning with the first reside of the initiation ATG codon)...In order to estimate the level of nonspecific bindings, we used a 100 molar excess of the cold antisense probes coexisting with the labeled probes. The cold antisense riboprobes were synthesized similarly with unlabeled nucleotides.
| Measurement 2:
Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: HINCII-ApaI fragment of residues 185-1483
Sequence species:
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: for NMDAR2B, the 1,869-bp HindIIISac1 fragment of residues 2,231-4,100...The cold antisense riboprobes were synthesized similarly with unlabeled nucleotides.
| Measurement 3:
Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: StuI-ApaI fragment of residues 414-1390
Sequence species:
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: for NMDAR2C, the 976-bp StuI-ApaI fragment of residues 414-1390;..The cold antisense riboprobes were synthesized similarly with unlabeled nucleotides.
| Measurement 4:
Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: HINDIII-Sac1 fragment of resiudes 2231-4100
Sequence species:
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: for NMDAR2B, the 1,869-bp HindIIISac1 fragment of residues 2,231-4,100...The cold antisense riboprobes were synthesized similarly with unlabeled nucleotides.
| Measurement 5:
Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: EcoRV1-SacI fragment of resiudes 2548-3824
Sequence species:
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: for NMDAR2D, the 1,276-bp EcoRV-Sac1 fragment of residues 2548-3824...The cold antisense riboprobes were synthesized similarly with unlabeled nucleotides.
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