Metadata

Experiment acronym Annotation Collator Author checked
Wada-nAChRDifferent cRNA probes were used in this study to map the distribution of the nACHR subunit mRNAs throughout the central nervous system of the map.Mihail Botano

Animals (subjects)
Sex: M
Number of animals: not specified
Age: not specified
Mass: 200-350
Unit of mass:
Housing conditions: normal housing
Annotation: Adult male rats (200-350 g) were anesthetized with 35% chloral hydrate and perfused transcardially as described previously (Swanson et al. 1983b).
Experimental method
Experiment type: in situ hybridization
Neuron/glia identification method: stain specific
Staining frequency: 1:5?

Experimental details:
Measured nucleic acid: mRNA
Source (producer): produced by authors
Probe sequence: PCA48
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: CRNA probes were prepared by using the in vitro transciption plasmids pSp65, pSP64, or pGEM. Collator note: see Table 1 page 317 for summary of the probles and histological series.

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: microtome Preservation: freezing Thickness: 25 micrometer
Staining type: cresyl violet Sampling: 1:5? Annotation: The brains were cut on a sliding microtome. Sections (25 micrometers thick) were mounted on poly-L-lysine-coated slides and air-dried under vaccum.

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
representative images with label and drawn boundaries
representative drawings or mappings onto templates
Mapping approach: brain region is captured in a BAMS nomenclature other than that used in the original publication