| Animals (subjects) | | Sex: M | | Number of animals: 5 | | Age: not specified | | Mass: not specified | | Unit of mass: not specified | | Housing conditions: normal housing | | Annotation: Sprague-Dawley rats (IFFA, Credo, France), housed under natural daylight conditions with food and water provided ad libitum, were used.Five adult male rats were deeply anesthetized and thenperfused through the ascending aorta with 150 ml 0.9%NaCl followed by 300 ml ice-cold 1% paraformaldehyde fixative in 0.1 M phosphate buffer (PBS; pH 7.4). |
| | Experimental method | | Experiment type: immunohistochemistry | | Neuron/glia identification method: not specified | | Staining frequency: 1:4 | 28, 1637
Experimental details:
| Antigen: Rat sulfhydryl oxidase
Antigen species: rat
Source (producer): Enzymologie et Chimie des Proteines Lab, Tours (France)
Primary antibody: rQSOX antiserum
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): polyclonal
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: immunoblot
Visualization method: fluoroscein
Visualization medium: slide
Annotation: The rQSOX antiserum used in this study was prepared in the laboratory of “Enzymologie et Chimie des Proteines,” Tours (France). Rat sulfhydryl oxidase was purified from seminal vesicle fluid (Benayoun et al., 2001); 6.5 micrograms of the purified protein in Freund’s complete adjuvant were subcutaneously inoculated to a rabbit at day 0. Injections of similar amounts of protein were repeated with incomplete adjuvant at days 21 and 71. Blood was collected from the ear marginal vein and the obtained antiserum was stored at –20°C.
The specificity of the rQSOX antiserum has been previously controlled by Western blot on seminal vesicle fluid homogenates (Benayoun et al., 2001).
| |
|
