| Animals (subjects) | | Sex: M | | Number of animals: 6 | | Age: not specified | | Mass: 280-320 | | Unit of mass: | | Housing conditions: normal housing | | Annotation: Adult male Sprague-Dawley rats (280-320 g BW at injection) were maintained on a 12 hour light/ 12 hour dark photoperiod (lights on 0700 hours) with water and rat chow available ad libitum. They were allowed 7 days' acclimatization to the animal quarters before we proceeded with the experiment. |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: stain specific | | Staining frequency: 1:8 |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): Promega Gemini
Probe sequence: cDNA AVP 1004
Sequence species: not specified
Probe sequence orientation: antisense
Control: sense
Labelling method: not specified
Visualization method: autoradiogram
Visualization medium: slide
Annotation: The pp- AVP sequence was generated from the cDNA AW 1004 described by Carrazana et al. (1988)....Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization..
| |
|
