| Animals (subjects) | | Sex: M | | Number of animals: 10 | | Age: not specified | | Mass: 250-300 | | Unit of mass: | | Housing conditions: normal housing | | Annotation: Male Sprague-Dawley rats (n = 10, 250–300 g; B&K Universal, Stockholm, Sweden) and male C57B1mice (n = 5, 20–40 g; B&K Universal) were used. The animals were kept under regular lighting conditions (lights on at 6.00 and off at 18.00) in a temperature-controlled environment and had free access to standard rodent chow and tap water. |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: stain specific | | Staining frequency: 1:10 |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): Scandinavian Gene Synthesis, Koping, Sweden).
Probe sequence: 479-527 MCH nucleotides
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Probes complementary to nucleotides 479-527 of the rat MCH mRNA(Nahon et al., 1989) and to nucleotides 2-49 of the rat H/O mRNA(Sakurai et al., 1998) were synthesized (Scandinavian Gene Synthesis, Ko¨ping, Sweden). Probe sequences were controlled against other sequences in the GenBank database, and no homologies exceeding 75% were found.
| | Measured nucleic acid: mRNA
Source (producer): Scandinavian Gene Synthesis, Koping, Sweden).
Probe sequence: 2-49 H/O nucleotides
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Probes complementary to nucleotides 479-527 of the rat MCH mRNA(Nahon et al., 1989) and to nucleotides 2-49 of the rat H/O mRNA(Sakurai et al., 1998) were synthesized (Scandinavian Gene Synthesis, Ko¨ping, Sweden). Probe sequences were controlled against other sequences in the GenBank database, and no homologies exceeding 75% were found.
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