| Animals (subjects) | | Sex: M | | Number of animals: 45 | | Age: not specified | | Mass: 300-250 | | Unit of mass: | | Housing conditions: normal housing | | Annotation: Forty-five male Sprague-Dawley rats (300-350 g) were housed in clear plasic cages in 12:12-h light-dark cycles (lights on at 07:00 h) with free access to Purina rat chow and water. |
| | Experimental method | | Experiment type: immunohistochemistry | | Neuron/glia identification method: stain specific | | Staining frequency: 1:10 | 25, 1951
Experimental details:
| Antigen: H/O
Antigen species: N/A
Source (producer): van der Pol
Primary antibody: hypocretin antiserum
Primary antibody species: not specified
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: Material for the analysis of the chemoarchitecture of the PHA was prepared by incubating coronal series (n=10) in antisera generated against neuropeptide Y (Peninsula, CA), met-enkephalin (Incstar, Stillwater, MN), tyrosine hydroxylase (Eugene Tech, Ramsey, NJ), gamma-aminobytyric acid (Eugene Tech), glutamic acid decarboxylase (Eugene Tech), glutamate (Sigma), 5-hydroxy-tryptamine (Incstar), dopamine-b-hydroxylase (Incstar), melanin concentrating hormone (Sawchenko), or hypocretin (van den Pol).
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