Animals (subjects) | Sex: M | Number of animals: 9 | Age: not specified | Mass: not specified | Unit of mass: not specified | Housing conditions: normal housing | Annotation: A total of 30 adult male rats, consisting of 4 different groups was used. The first was made up of 13 normal male Sprague-Dawley rats. 5 of them received colchicine into the lateral cerebral ventricle, 4 received intracisternal colchicine. |
| Experimental method | Experiment type: immunohistochemistry | Neuron/glia identification method: stain specific | Staining frequency: 1:5 or 1:10 | 30, 2068 Experimental details:
| Antigen: AII Antigen species: not specified Source (producer): specified elsewhere Primary antibody: Rabbit DE Primary antibody species: rabbit Antibody type (monoclonal, polyclonal): not specified Secondary antibody: not specified Secondary antibody species: goat Immunoglobulin class: Alpa globulin Control: adsorption Visualization method: fluorescence Visualization medium: slide Annotation: The production and characterization of the AII antiserum (Rabbit DE) are described in detail elsewhere [8,58]. For all of our experiments it was used at a dilution of 1:2,500 and the secondary antiserum, affinity-purified goat-anti-rabbit conjugated to fluorescein isothycyanate (Tago), was used at a dilution of 1:200. To demonstrate specificity of immunohistochemical staining, all of the major cell groups and fiber systems described below were examined with anitserum that was blocked by liquid-phase absorption. The same solid-pahse adsorption technique was used to demonstrate an absence of crossreactivity between the anti-AII serum and vasopressin in the hypothalamus and basal telencephalon.
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