Metadata

Experiment acronym Annotation Collator Author checked
Risold-LSIn situ hybridization and immunohistochemistry study of the chemoarchitecture pattern of the lateral septum. The reference does not provide enough information about procedure followed for each investigated molecule, therefore we grouped all data into a single experiment with several measurements.Mihail Botano

Animals (subjects)
Sex: M
Number of animals: not specified
Age: not specified
Mass: not specified
Unit of mass: not specified
Housing conditions: normal housing
Annotation: All animals were adult male Sprague–Dawley rats.
Experimental method
Experiment type: in situ hybridization
Neuron/glia identification method: stain specific
Staining frequency: 1:4

Experimental details:
Measured nucleic acid: mRNA
Source (producer): see Escalpez et al, 1993
Probe sequence: enkephalin
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Collator note: see associated reference for technique details, and Table 1 page 93.

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: microtome Preservation: freezing Thickness: 15 micrometer
Staining type: cresyl violet Sampling: 1:4 Annotation: Most of the in situ hybridization material used in this study is from a library of closely spaced 1-in-4, 15 micrometers thick frozen sections through the rat forebrain, treated with probes for the major neuropeptides, as well as for certain proteins that are expressed in the brain.

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
representative images with label and drawn boundaries
Mapping approach: brain region is captured in a BAMS nomenclature other than that used in the original publication