Metadata

Experiment acronym Annotation Collator Author checked
Sawchenko-Swanson-1982This experiment combines measurements of several molecules in the rat PVH.Mihail Botano

Animals (subjects)
Sex: M
Number of animals: not specified
Age: not specified
Mass: 275-325
Unit of mass:
Housing conditions: normal housing
Annotation: All experiments were carried out in adult male Sprague-Dawley albino rats which weighed approximately 275-325 gm and which had unrestricted access to food and water throughout the course of the experiments.
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Experimental method
Experiment type: immunohistochemistry
Neuron/glia identification method: stain specific
Staining frequency: not specified

Experimental details:
Antigen: not specified
Antigen species: not specified
Source (producer): Antivassopressin antiserum, donated by Drs. K. Dierickx & Vandensande
Primary antibody: not specified
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: adsorption
Visualization method: fluoroscein
Visualization medium: slide
Annotation: Collator note: see Materials and methods Section, page 261.

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: microtome Preservation: freezing Thickness: 20 micrometer
Staining type: cresyl violet Sampling: 1:5 Annotation: The brains were removed immediately and were placed in the same fixative (with 15% sucrose) at 4 Celsius degrees, where they remained overnight. The following day, five adjacent one-in-five series of 20-micrometers-thick frozen sections were cut on a sliding microtome. One series from each brain was stained with thionin and was to make projection drawings of relevant cell groups in the hypothalamus.

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
drawings or template mappings of all sections
Mapping approach: brain region is captured in a BAMS nomenclature other than that used in the original publication