| Animals (subjects) | | Sex: M | | Number of animals: not specified | | Age: not specified | | Mass: not specified | | Unit of mass: not specified | | Housing conditions: normal housing | | Annotation: All animals were adult male Sprague–Dawley rats. |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: stain specific | | Staining frequency: 1:4 |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: mineralocorticoid receptor
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: For the in situ hybridization of androgen, estrogen, and mineralocorticoid receptor mRNAs, original material from publications reporting their distribution in the rat brain were re-examined [3,98] . Some of this material was kindly provided by Dr. R.B. Simerly, whom the authors also wish to thank. For details about the methods used to prepare this material, readers should consult to the original publications.
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