| Experimental method |
| Experiment type: in situ hybridization |
| Neuron/glia identification method: stain specific |
| Staining frequency: 1:4 |
Experimental details:
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Measured nucleic acid: mRNA
Source (producer): see Escalpez et al, 1993
Probe sequence: enkephalin
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Collator note: see associated reference for technique details, and Table 1 page 93.
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Measured nucleic acid: mRNA
Source (producer): see Kislaukis et al. 1988
Probe sequence: neurotensin
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Collator note: see associated reference for technique details, and Table 1 page 93.
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Measured nucleic acid: mRNA
Source (producer): see Goodman et al. 1983
Probe sequence: somatostatin
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Collator note: see associated reference for technique details, and Table 1 page 93.
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Measured nucleic acid: mRNA
Source (producer): see Civelly et al. 185
Probe sequence: preprodynorphin
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Collator note: see associated reference for technique details, and Table 1 page 93.
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Measured nucleic acid: mRNA
Source (producer): see Mayo et al. 1985
Probe sequence: GRH
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Collator note: see associated reference for technique details, and Table 1 page 93.
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Measured nucleic acid: mRNA
Source (producer): see Krause et al. 1987
Probe sequence: substance P
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Collator note: see associated reference for technique details, and Table 1 page 93.
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Measured nucleic acid: mRNA
Source (producer): see Larhammar et al. 1987
Probe sequence: neuropeptide Y
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Collator note: see associated reference for technique details, and Table 1 page 93.
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Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: mineralocorticoid receptor
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: For the in situ hybridization of androgen, estrogen, and mineralocorticoid receptor mRNAs, original material from publications reporting their distribution in the rat brain were re-examined [3,98] . Some of this material was kindly provided by Dr. R.B. Simerly, whom the authors also wish to thank. For details about the methods used to prepare this material, readers should consult to the original publications.
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Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: androgen receptor
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: For the in situ hybridization of androgen, estrogen, and mineralocorticoid receptor mRNAs, original material from publications reporting their distribution in the rat brain were re-examined [3,98] . Some of this material was kindly provided by Dr. R.B. Simerly, whom the authors also wish to thank. For details about the methods used to prepare this material, readers should consult to the original publications.
|
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Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: estrogen receptor
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: For the in situ hybridization of androgen, estrogen, and mineralocorticoid receptor mRNAs, original material from publications reporting their distribution in the rat brain were re-examined [3,98] . Some of this material was kindly provided by Dr. R.B. Simerly, whom the authors also wish to thank. For details about the methods used to prepare this material, readers should consult to the original publications.
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