| Animals (subjects) | | Sex: M | | Number of animals: not specified | | Age: not specified | | Mass: 150-250 | | Unit of mass: | | Housing conditions: normal housing | | Annotation: A total of 65 male, specific pathogen-free Sprague-Dawley albino rats (Anticimex AB, Stockholm, Sweden) was used.Different postnatal stages (0, 1, 2, 3, 5, 7, 9, 12, 14, 21, 30 days) and adult rats (150-200 g body weigh) were studied. |
| | Experimental method | | Experiment type: immunohistochemistry | | Neuron/glia identification method: morphology | | Staining frequency: 1:2 | 14, 3485
Experimental details:
| Antigen: cyclic SOM 25-28
Antigen species: synthetic
Source (producer): not specified
Primary antibody: antibody 8
Primary antibody species: mouse
Antibody type (monoclonal, polyclonal): monoclonal
Secondary antibody: anti-mouse antibody
Secondary antibody species: goat/sheep
Immunoglobulin class: not specified
Control: staining pattern
Visualization method: fluoroscein
Visualization medium: slide
Annotation: ... mouse monoclonal antibodies against synthetic cyclic somatostatin 15-28 conjugated to keyhole limpet hemocyanine (antibody 8, Buchan et al. 1985) were applied in a dilution of 30 micrograms protein/ml. .the sections were... incubated with fluoresceine isothiocyanate (FITC) conjugated to goat (1:40; Boehringer Mannheim Scandinavia, Stockholm, Sweden) or sheep (1:10, Amersham, Little Chalfont, England) anti-mouse antibodies for 30 min at 37C. In addition control experiments using sections of the
cerebellum at different stages of development were performed by incubation with antibodies preabsorbed with 10 micrograms SOM 15-28 peptide (Peninsula Labs, Belmont, CA, USA) per ml diluted antibody.
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