| Animals (subjects) | | Sex: M | | Number of animals: 3 | | Age: not specified | | Mass: 150 | | Unit of mass: | | Housing conditions: not specified | | Annotation: Three male Wistar rats (Kiwa Experimental Animal Company, Japan) weighing c. I50 g were decapitated under sodium pentobarbital anesthesia (50 mg/kg, ip.). |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: morphology | | Staining frequency: not specified |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: not specified
Sequence species: not specified
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: colorimetry
Visualization medium: film
Annotation: Collator note: information about the procedure used for in situ hybridization was described in Noguchi et al. 1988 and Keinanen et al. 1990.
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