| Animals (subjects) | | Sex: M/F | | Number of animals: 16 | | Age: not specified | | Mass: 100-300 | | Unit of mass: | | Housing conditions: normal housing | | Annotation: A total of 16 young and adult Sprague-Dawley rats of either sex, 100-300 gin body weight (b.w.1, purchased from approved breeders, were used for this investigation. The animals were housed and handled according to guidelines supervised by an institutional animal care and usage committee. |
| | Experimental method | | Experiment type: immunohistochemistry | | Neuron/glia identification method: morphology | | Staining frequency: not specified | 16, 3536
Experimental details:
| Antigen: NR1 c-term
Antigen species: rat
Source (producer): not specified
Primary antibody: AbT3-1
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: anti rabbit
Secondary antibody species: goat
Immunoglobulin class: IgG
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: The primary antibodies employed in this study have been extensively characterized in previous reports, and their specificity has been determined (Petralia and Wenthold, 1992; Wenthold et al., 1992, 1994; Brose et al., 1993; Huntley et al., 1993; Petralia et al., 1994a,b; Siegel et al., 1994). AbT3-1 is a rabbit antiserum raised against the carboxy-terminus of NR1 (Petralia et al., 1994a) and recognizes the NR1-splice variants without 3’-end deletion 2, i.e., NR1-la, -2a, -lb, -2b (Sucher et al., 1992; Hollman et al., 1993; Petralia et al., 1994a). Goat anti-rabbit and goat anti-mouse sera and rabbit and mouse PAP were purchased from Sternberger Monoclonals Inc. (Baltimore, MD). Collator note: see Table 1 page 147 for details.
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