| Animals (subjects) | | Sex: M | | Number of animals: not specified | | Age: not specified | | Mass: 200-300 | | Unit of mass: | | Housing conditions: not specified | | Annotation: Adult male Sprague-Dawley rats (200-300 g) and pups were deeply anesthetized with ether and then sacrificed by decapitation. At least three animals of each age were used for in situ hybridization histochemistry. |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: morphology | | Staining frequency: not specified |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: HINCII-ApaI fragment of residues 185-1483
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: for NMDAR2B, the 1,869-bp HindIIISac1 fragment of residues 2,231-4,100...The cold antisense riboprobes were synthesized similarly with unlabeled nucleotides.
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