| Animals (subjects) | | Sex: M | | Number of animals: not specified | | Age: not specified | | Mass: 200-250 | | Unit of mass: | | Housing conditions: not specified | | Annotation: Adult male Sprague-Dawley rats (Charles River, 200-250g) were killed by decapitation and their brains quickly removed and frozen in liquid isopentane (- 30°C, 30s). |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: morphology | | Staining frequency: not specified |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: D1 receptor fragment
Sequence species: not specified
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: Brain sections were hybridized with a [35S]uridine triphosphate (UTP)-labeled 480-bp riboprobe generated to the region of the rat D1, receptor spanning transmembranesIII-VI (base pairs 383-843). No specific hybridization was observed with sections
labeled with the sense-strand probe, while the antisense riboprobe showed specific hybridization.
| |
|
