| Animals (subjects) | | Sex: M | | Number of animals: not specified | | Age: not specified | | Mass: 180-200 | | Unit of mass: | | Housing conditions: not specified | | Annotation: Male Wistar rats (weighing 180-200g; IFFA Credo, France) were decapitated, the brain rapidly removed and immediately frozen (-40°C) by immersion in liquid monochlorodifluoromethane. |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: morphology | | Staining frequency: not specified |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): Promega
Probe sequence: Bam H1-Ava I restriction fragment
Sequence species: not specified
Probe sequence orientation: not specified
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: The D 3 receptor m RNA probe consisted of a Barn H I-Ava I restriction fragment, corresponding to the third intracytoplasmic loop of the receptor, and has been described previously. Single-stranded RNA probes were synthesized using
a Riboprobe kit (Promega) with [32P]UTP, [33P]UTP, [35S]UTP or a mixture of digoxigenin-UTP and non-labeled UTP (75:25) with T7 or SP6 RNA polymerase and linearized recombinant plasmids in the proper orientation (10 ng) as templates.
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