| Animals (subjects) | | Sex: M/F | | Number of animals: 15 | | Age: not specified | | Mass: 250-400 | | Unit of mass: | | Housing conditions: normal housing | | Annotation: The materials for this study are derived from 15 adult male and
female Sprague-Dawley rats, 250-400 g in body weight. The animals
were housed in supervised animal facilities and handled according
to approved guidelines. |
| | Experimental method | | Experiment type: immunohistochemistry | | Neuron/glia identification method: morphology | | Staining frequency: not specified | 21, 3721
Experimental details:
| Antigen: CART
Antigen species: not specified
Source (producer): not specified
Primary antibody: CART antiserum
Primary antibody species: not specified
Antibody type (monoclonal, polyclonal): monoclonal
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: staining pattern
Visualization method: colorimetry
Visualization medium: slide
Annotation: Some of the immunoreacted sections were
postfixed for 3 min in 0.005% osmium tetroxide in water to enhance
contrast. Control sections incubated with preimmune
rabbit serum were completely free of immunoreaction product, as
previously documented (Winsky and Jacobowitz 1991).
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