| Animals (subjects) | | Sex: M | | Number of animals: not specified | | Age: not specified | | Mass: 200-350 | | Unit of mass: | | Housing conditions: normal housing | | Annotation: Adult male rats (200-350 g) were anesthetized with 35% chloral hydrate and perfused transcardially as described previously (Swanson et al. 1983b). |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: stain specific | | Staining frequency: 1:5? |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): produced by authors
Probe sequence: C183
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: CRNA probes were prepared by using the in vitro transciption plasmids pSp65, pSP64, or pGEM. Collator note: see Table 1 page 317 for summary of the probles and histological series.
| | Measured nucleic acid: mRNA
Source (producer): produced by authors
Probe sequence: PCA48
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: CRNA probes were prepared by using the in vitro transciption plasmids pSp65, pSP64, or pGEM. Collator note: see Table 1 page 317 for summary of the probles and histological series.
| | Measured nucleic acid: mRNA
Source (producer): produced by authors
Probe sequence: HYA23-1
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: CRNA probes were prepared by using the in vitro transciption plasmids pSp65, pSP64, or pGEM. Collator note: see Table 1 page 317 for summary of the probles and histological series.
| | Measured nucleic acid: mRNA
Source (producer): produced by authors
Probe sequence: HYA11
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: CRNA probes were prepared by using the in vitro transciption plasmids pSp65, pSP64, or pGEM. Collator note: see Table 1 page 317 for summary of the probles and histological series.
| | Measured nucleic acid: mRNA
Source (producer): produced by authors
Probe sequence: PCX49
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: film
Annotation: CRNA probes were prepared by using the in vitro transciption plasmids pSp65, pSP64, or pGEM. Collator note: see Table 1 page 317 for summary of the probles and histological series.
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