| Animals (subjects) | | Sex: M | | Number of animals: 3 | | Age: not specified | | Mass: not specified | | Unit of mass: not specified | | Housing conditions: normal housing | | Annotation: The third group was bilaterally adrenalectomized and allowed to survive for 3 days, 1 month and 2 months before perfusion (n=3). |
| | Experimental method | | Experiment type: immunohistochemistry | | Neuron/glia identification method: not specified | | Staining frequency: 1:4 or 1:8 | 9, 61
Experimental details:
| Antigen: CRF
Antigen species: sheep
Source (producer): not specified
Primary antibody: 24 ([Try22. Gly23]-CRF (1-23)
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): polyclonal
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: Alpha globulin
Control: not specified
Visualization method: fluorescence
Visualization medium: slide
Annotation: Seven different antisera to synthetic ovine CRF (or to a fragment of CRF), which were raised in rabbits [7], were tested and all but one were found to stain cells and fibers in the rat. Since antiserum 24 ([Try22. Gly23]-CRF (1-23) conjugated to human alpha-globulin with bis-diazetized benzidine] has been found to interact with rat CRF particularly well in radioimmunoassays [Vale, unpublished observations], it was used extensively for the mapping studies reported here.
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