Metadata

Experiment acronym Annotation Collator Author checked
Watts-NT/pENK (PEG)NT/ENK coexpression in the PVHmpd in PEG administration conditionMihail Botano

Animals (subjects)
Sex: M
Number of animals: 3
Age: not specified
Mass: 280-320
Unit of mass:
Housing conditions: normal housing
Annotation: Adult male Sprague-Dawley rats (280-320 g BW at injection) were maintained on a 12 hour light/ 12 hour dark photoperiod (lights on 0700 hours) with water and rat chow available ad libitum. They were allowed 7 days' acclimatization to the animal quarters before we proceeded with the experiment.
Experimental method
Experiment type: in situ hybridization
Neuron/glia identification method: stain specific
Staining frequency: 1:8

Experimental details:
Measured nucleic acid: mRNA
Source (producer): not specified
Probe sequence: pp-neurotensin/neuromedin
Sequence species: not specified
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Sections were hybridized with 35SUTP- labelled complementary RNA (cRNA) probes transcribed from cDNA sequences for part of the mRNAs encoding pp-neurotensin/neuromedin (pENK)....All 35S-UTP probes were synthesized using the Riboprobe Gemini System I1 kit (Promega Inc., Madison, WI) and the appropriate RNA polymerase using a modification of the method of Melton et al. (1984)...

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: microtome Preservation: freezing Thickness: 15 micrometer
Staining type: thionin Sampling: 1:8 Annotation: Eight series of one-in-eight, 15 micrometers thick frontal sections were cut through the hypothalamus...

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
representative drawings or mappings onto templates
Mapping approach: brain region is captured in a BAMS nomenclature other than that used in the original publication