Metadata

Experiment acronym Annotation Collator Author checked
Jaarma-GluIn situ hybridization study of different glutamate receptor subunits expression in the rat and cat cerebellar cortex.Mihail Botano

Animals (subjects)
Sex: M/F
Number of animals: 16
Age: not specified
Mass: 100-300
Unit of mass:
Housing conditions: normal housing
Annotation: A total of 16 young and adult Sprague-Dawley rats of either sex, 100-300 gin body weight (b.w.1, purchased from approved breeders, were used for this investigation. The animals were housed and handled according to guidelines supervised by an institutional animal care and usage committee.
16, 3536
Experimental method
Experiment type: immunohistochemistry
Neuron/glia identification method: morphology
Staining frequency: not specified

Experimental details:
Antigen: NR1 c-term
Antigen species: rat
Source (producer): not specified
Primary antibody: AbT3-1
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: anti rabbit
Secondary antibody species: goat
Immunoglobulin class: IgG
Control: not specified
Visualization method: colorimetry
Visualization medium: slide
Annotation: The primary antibodies employed in this study have been extensively characterized in previous reports, and their specificity has been determined (Petralia and Wenthold, 1992; Wenthold et al., 1992, 1994; Brose et al., 1993; Huntley et al., 1993; Petralia et al., 1994a,b; Siegel et al., 1994). AbT3-1 is a rabbit antiserum raised against the carboxy-terminus of NR1 (Petralia et al., 1994a) and recognizes the NR1-splice variants without 3’-end deletion 2, i.e., NR1-la, -2a, -lb, -2b (Sucher et al., 1992; Hollman et al., 1993; Petralia et al., 1994a). Goat anti-rabbit and goat anti-mouse sera and rabbit and mouse PAP were purchased from Sternberger Monoclonals Inc. (Baltimore, MD). Collator note: see Table 1 page 147 for details.

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: microtome Preservation: freezing Thickness: 30 micrometer
Staining type: none Sampling: not specified Annotation: Frozen sections were cut at 30 micrometers on a sliding microtome in the sagittal or coronal planes and were collected in ice-cold phosphate buffer. Free-floating sections were processed for immunocytochemistry using the peroxidase-antiperoxidase (PAP) or the avidin-biotinylated peroxidase-complex (ABC) protocols with 3,3‘-diaminobenzidine tetrahydrochloride (DAB) as the chromogen.

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
Mapping approach: brain region is captured in a BAMS nomenclature other than that used in the original publication