Animals (subjects) | Sex: M | Number of animals: 10 | Age: not specified | Mass: 250-300 | Unit of mass: | Housing conditions: normal housing | Annotation: Male Sprague-Dawley rats (n = 10, 250–300 g; B&K Universal, Stockholm, Sweden) and male C57B1mice (n = 5, 20–40 g; B&K Universal) were used. The animals were kept under regular lighting conditions (lights on at 6.00 and off at 18.00) in a temperature-controlled environment and had free access to standard rodent chow and tap water. |
| Experimental method | Experiment type: in situ hybridization | Neuron/glia identification method: stain specific | Staining frequency: 1:10 | Experimental details:
| Measured nucleic acid: mRNA Source (producer): Scandinavian Gene Synthesis, Koping, Sweden). Probe sequence: 479-527 MCH nucleotides Sequence species: rat Probe sequence orientation: antisense Control: sense Labelling method: radiolabelling Visualization method: autoradiogram Visualization medium: slide Annotation: Probes complementary to nucleotides 479-527 of the rat MCH mRNA(Nahon et al., 1989) and to nucleotides 2-49 of the rat H/O mRNA(Sakurai et al., 1998) were synthesized (Scandinavian Gene Synthesis, Ko¨ping, Sweden). Probe sequences were controlled against other sequences in the GenBank database, and no homologies exceeding 75% were found.
| | Measured nucleic acid: mRNA Source (producer): Scandinavian Gene Synthesis, Koping, Sweden). Probe sequence: 2-49 H/O nucleotides Sequence species: rat Probe sequence orientation: antisense Control: sense Labelling method: radiolabelling Visualization method: autoradiogram Visualization medium: slide Annotation: Probes complementary to nucleotides 479-527 of the rat MCH mRNA(Nahon et al., 1989) and to nucleotides 2-49 of the rat H/O mRNA(Sakurai et al., 1998) were synthesized (Scandinavian Gene Synthesis, Ko¨ping, Sweden). Probe sequences were controlled against other sequences in the GenBank database, and no homologies exceeding 75% were found.
| |
|