Metadata

Experiment acronym Annotation Collator Author checked
Sawchenko-1984CRH-oxytocin colocalization study in the rat PVH.Mihail Botano

Animals (subjects)
Sex: M
Number of animals: not specified
Age: not specified
Mass: not specified
Unit of mass: not specified
Housing conditions: normal housing
Annotation: Adult male albino rats of the Sprague-Dawley strain were used in all experiments.
27, 1408
Experimental method
Experiment type: immunohistochemistry
Neuron/glia identification method: stain specific
Staining frequency: 1:5

Experimental details:

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: not specified Preservation: freezing Thickness: 20 micrometer
Staining type: thionin Sampling: 1:5 Annotation: Up to five one-in-five series of 20 micrometers thick frozen sections through the PVH were saved and then prepared for indirect immunofluorescence staining of cells that cross-react with antisera against CRF, neurotensin, oxytocin, or vasopressin using a conventional method (Sawchenko and Swanson, 1981) based upon the localization of a primary antiserum raised in rabbits using an affinity-purified, fluorescein-conjugated goat anti-rabbit IgG. The distributions of labeled cells were mapped onto projection drawings made from adjacent series of sections counterstained with thionin, and the region around the PVH was photographed at x 100 magnification using Ilford XP-1 film.

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
representative drawings or mappings onto templates
Mapping approach: brain region is captured in a BAMS nomenclature other than that used in the original publication