Metadata

Experiment acronym Annotation Collator Author checked
Coello-QSOXImmunohistochemistry mapping study of the sulfhydryl oxidase QSOX in the rat brain. Mihail Botano

Animals (subjects)
Sex: M
Number of animals: 5
Age: not specified
Mass: not specified
Unit of mass: not specified
Housing conditions: normal housing
Annotation: Sprague-Dawley rats (IFFA, Credo, France), housed under natural daylight conditions with food and water provided ad libitum, were used.Five adult male rats were deeply anesthetized and thenperfused through the ascending aorta with 150 ml 0.9%NaCl followed by 300 ml ice-cold 1% paraformaldehyde fixative in 0.1 M phosphate buffer (PBS; pH 7.4).
28, 1576
Experimental method
Experiment type: immunohistochemistry
Neuron/glia identification method: not specified
Staining frequency: 1:4

Experimental details:
Antigen: Rat sulfhydryl oxidase
Antigen species: rat
Source (producer): Enzymologie et Chimie des Proteines Lab, Tours (France)
Primary antibody: rQSOX antiserum
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): polyclonal
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: immunoblot
Visualization method: fluoroscein
Visualization medium: slide
Annotation: The rQSOX antiserum used in this study was prepared in the laboratory of “Enzymologie et Chimie des Proteines,” Tours (France). Rat sulfhydryl oxidase was purified from seminal vesicle fluid (Benayoun et al., 2001); 6.5 micrograms of the purified protein in Freund’s complete adjuvant were subcutaneously inoculated to a rabbit at day 0. Injections of similar amounts of protein were repeated with incomplete adjuvant at days 21 and 71. Blood was collected from the ear marginal vein and the obtained antiserum was stored at –20°C. The specificity of the rQSOX antiserum has been previously controlled by Western blot on seminal vesicle fluid homogenates (Benayoun et al., 2001).

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: microtome Preservation: freezing Thickness: 20 micrometer
Staining type: not specified Sampling: 1:4 Annotation: Five adult male rats were deeply anesthetized and then perfused through the ascending aorta with 150 ml 0.9% NaCl followed by 300 ml ice-cold 1% paraformaldehyde fixative in 0.1 M phosphate buffer (PBS; pH 7.4). The brains were immediately removed, postfixed in the same fixative for 2 hours at 4°C, immersed overnight at 4°C in a 15% cryoprotective sucrose solution, and quickly frozen over liquid nitrogen. Then they were serially cut into 20-micrometers coronal sections on a cryostat-microtome, mounted on gelatinized slides and stored at –40°C until treatment. Two brains were used for the mapping of the rQSOX distribution on every four sections from the olfactory bulbs to the spinal cord...

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
representative drawings or mappings onto templates
Mapping approach: brain region is captured in original published nomenclature, in BAMS