Metadata

Experiment acronym Annotation Collator Author checked
Watts-CRH (PEG)CRH mRNA, CRH hnRNA, pENK mRNA and c-fos mRNA were measured in PVH.Mihail Botano

Animals (subjects)
Sex: M
Number of animals: not specified
Age: not specified
Mass: 225-250
Unit of mass:
Housing conditions: normal housing
Annotation: Adult male Sprague Dawley rats (225-250 gm body weight at the beginning of the experiment) were maintained on a 12 hr light/dark photoperiod (lights on at 6:00 AM) with ad libitum access to water and rat chow and were allowed at least 5d of acclimation to the animal quarters.
Experimental method
Experiment type: in situ hybridization
Neuron/glia identification method: stain specific
Staining frequency: 1:8

Experimental details:
Measured nucleic acid: mRNA
Source (producer): Promega Gemini
Probe sequence: preproCRH
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: not specified
Visualization method: autoradiogram
Visualization medium: film
Annotation: Sections were hybridized with 35S-UTP-labeled cRNA probes transcribed from cDNA sequences encoding RNAs for parts of the following genes: preproCRH (700 bp)...

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: not specified Preservation: freezing Thickness: 15 micrometer
Staining type: thionin Sampling: 1:8 Annotation: Eight series of one-in-eight, 15 micrometers thick frontal sections were cut through the hypothalamus...

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
Mapping approach: brain region is captured in original published nomenclature, in BAMS