Metadata

Experiment acronym Annotation Collator Author checked
Watts-CRH/NTCRH/NT coexpression was measured in normal conditions.Mihail Botano

Animals (subjects)
Sex: M
Number of animals: 5
Age: not specified
Mass: 280-320
Unit of mass:
Housing conditions: normal housing
Annotation: Adult male Sprague-Dawley rats (280-320 g BW at injection) were maintained on a 12 hour light/ 12 hour dark photoperiod (lights on 0700 hours) with water and rat chow available ad libitum. They were allowed 7 days' acclimatization to the animal quarters before we proceeded with the experiment.
Experimental method
Experiment type: in situ hybridization
Neuron/glia identification method: stain specific
Staining frequency: 1:8

Experimental details:
Measured nucleic acid: mRNA
Source (producer): Promega Gemini
Probe sequence: pp-neurotensin/neuromedin
Sequence species: not specified
Probe sequence orientation: antisense
Control: sense
Labelling method: not specified
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Sections were hybridized with 35S UTP- labelled complementary RNA (cRNA) probes transcribed from cDNA sequences for part of the mRNAs encoding pp-neurotensin/neuromedin N (NT/N), or pp-vasopressin...Details of the probes and their characterization have been given in publications from this and other laboratories (Carrazana et al., 1988; Swanson and Simmons, 1989; Frim et al., 1990; Watts, 1992b).. Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization...All 35S-UTP probes were synthesized using the Riboprobe Gemini System I1 kit (Promega Inc., Madison, WI) and the appropriate RNA polymerase using a modification of the method of Melton et al. (1984)

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: microtome Preservation: freezing Thickness: 15 micrometer
Staining type: thionin Sampling: 1:8 Annotation: Eight series of 1-in-8 15-pm-thick coronal sections were cut through the hypothalamus and saved in ice-cold potassium phosphate-buffered saline (KPBS, pH 7.4) containing 0.25% paraformaldehyde....Serial sections were saved for thionin staining.

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
representative drawings or mappings onto templates
Mapping approach: brain region is captured in original published nomenclature, in BAMS