Metadata

Experiment acronym Annotation Collator Author checked
PS439Mapping of CRH neurons in the ardrenalectomized rat brain. Mihail Botano

Animals (subjects)
Sex: M
Number of animals: 3
Age: not specified
Mass: not specified
Unit of mass: not specified
Housing conditions: normal housing
Annotation: The third group was bilaterally adrenalectomized and allowed to survive for 3 days, 1 month and 2 months before perfusion (n=3).
9, 60
Experimental method
Experiment type: immunohistochemistry
Neuron/glia identification method: not specified
Staining frequency: 1:4 or 1:8

Experimental details:
Antigen: CRF
Antigen species: sheep
Source (producer): not specified
Primary antibody: 24 ([Try22. Gly23]-CRF (1-23)
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): polyclonal
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: Alpha globulin
Control: not specified
Visualization method: fluorescence
Visualization medium: slide
Annotation: Seven different antisera to synthetic ovine CRF (or to a fragment of CRF), which were raised in rabbits [7], were tested and all but one were found to stain cells and fibers in the rat. Since antiserum 24 ([Try22. Gly23]-CRF (1-23) conjugated to human alpha-globulin with bis-diazetized benzidine] has been found to interact with rat CRF particularly well in radioimmunoassays [Vale, unpublished observations], it was used extensively for the mapping studies reported here.

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: not specified Preservation: freezing Thickness: 30 micrometer
Staining type: thionin Sampling: 1:4 or 1:8 Annotation: In general, 1-in-4 or 1-in-8 serios of sections through the brain were stained for immunofluorescence. In addition, an adjacent series of sections from each brain was cut and stained with thionine.

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
drawings or template mappings of all sections
Mapping approach: brain region is captured in a BAMS nomenclature other than that used in the original publication